Non-standard equipment

• ESCO CelCulture CO2/O2 incubator (ESCO, USA) In vitro cell culture incubator with hypoxia function - controls temperature, humidity, carbon dioxide levels from 0% to 20% and oxygen concentration from 1% to 20.7% and humidity up to 97%; enables in vitro culture experimental work in cell biology, microbiology and molecular biology; applications also include tissue engineering, in vitro fertilization, neuroscience, cancer research and more 
  • a) general conditions of use - on a cooperative basis by agreement on the mode and duration of use
    b) location of equipment and contact person: Department of Cell Biology and Imaging, cell culture laboratory, Dr. Grzegorz Tylko, Prof. UJ; grzegorz.tylko@uj.edu.pl 
• Stationary, dual-gas CO2/O2 incubator for cell culture under hypoxia (0.1% to 21% O2 concentration), model Galaxy 48R from the New Brunswick Company. 
  • a) general conditions of use - on a cooperative basis by agreement on the mode and duration of use 
    b) location of equipment and contact person: Department of Cell Biology and Imaging, cell culture laboratory, Dr Marek Romek, Prof. UJ; marek.romek@uj.edu.pl.
• CytoSMART LUX2 (the Netherlands) - cell culture image recordings over a specified time interval from minutes to weeks; automatic notifications, graphical representation of cell division data, growth monitoring and culture confluence, allows spatial and temporal tracking of cell division and growth, colony formation, migration, chemotaxis, etc.; more information
  • a) general conditions of use - on a cooperative basis by agreement on the mode and duration of use, access to the online calendar of the equipment upon prior notification
    b) location of the equipment and contact person (contact details): Department of Cell Biology and Imaging, cell culture laboratory, Dr. Grzegorz Tylko, Prof. UJ, grzegorz.tylko@uj.edu.pl 
• LUX3TM FL (Axion BioSystems) - recording images of cell cultures over a specified time interval from minutes to weeks; automatic notifications, graphical representation of cell division data, growth monitoring and culture confluence, allows spatial and temporal tracking of cell division and growth, colony formation, migration, chemotaxis, etc. Two devices in fluorescence version with the possibility to observe cells transfected with fluorochromes in the green and red emission range; more information
  • a) general conditions of use - on a cooperative basis by agreement on the mode and time of use, access to the online calendar of the equipment upon prior notification.
    b) Location of equipment and contact person (contact details): Department of Cell Biology and Imaging, cell culture laboratory, Dr Grzegorz Tylko, Prof. UJ, grzegorz.tylko@uj.edu.pl. 
• EBERS TEB-1000 Bioreactor (EBERS, Spain) - Cell culture incubator with 2 built-in perfusion pumps for controlled, uni- or bidirectional fluid flow in a closed system in 2- and 3-dimensional cell cultures; more information
  • a) general conditions of use - On a cooperative basis by agreement on the mode and duration of use  
    b) Location of equipment and contact person (contact details): Department of Cell Biology and Imaging, cell culture laboratory, Prof. Dr. Anna M. Osyczka, anna.osyczka@uj.edu.pl 
• 4D-Nucleofector Y Unit with Core Unit (Lonza) - enables electroporation (insertion of DNA, RNA and related products into the cytoplasm and nucleus of cells) of adherent cells; see also
  • a) general terms of use - On the basis of cooperation after agreeing on the mode and time of use 
  • b) location of equipment and contact person (contact details): Department of Cell Biology and Imaging, cell culture laboratory, Prof. Anna M. Osyczka, anna.osyczka@uj.edu.pl 
• Drosophila melanogaster culture facility - Professional animal facility with D. melanogaster culture, plates for phenotyping and screening, stereo fluorescence microscope - screening for GFP, range of transgenic and mutant strains available 
  • a) general terms of use - On a cooperative basis after agreement on the mode and duration of use  
    b) Location of equipment and contact person (contact details) - Department of Cell Biology and Imaging, D. melanogaster culture laboratory, Dr. Milena Damulewicz, milena.damulewicz@uj.edu.pl, Dr. Jolanta Górska-Andrzejak, j.gorska-andrzejak@uj.edu.pl.
• Insect locomotor analysis system (DAMS) - Measurement of insect locomotor activity, sleep analysis under different light and temperature conditions
  • a) general terms of use - on a collaborative/agreed basis 
    b) Location of equipment and contact person (contact details) - Department of Cell Biology and Imaging, D. melanogaster culture laboratory Dr. Milena Damulewicz, milena.damulewicz@uj.edu.pl, Dr. Jolanta Górska-Andrzejak, j.gorska-andrzejak@uj.edu.pl.
• Zeiss AxioImager.2 with Apotome 3 attachment - allows the use of the SIM (Structured Illumination Microscopy) technique, providing a three-dimensional image of tissues and cells analogous to the images obtained with confocal microscopes..
  • a) general terms of use - on a collaborative/agreed basis
    b) Location of equipment and contact person (contact details) - Department of Cell Biology and Imaging, D. melanogaster culture laboratory, Dr. Milena Damulewicz, milena.damulewicz@uj.edu.pl.
• Electron and confocal microscopy laboratory – more
• ROTOFIX 32A Hettich laboratory centrifuge
  • a) general terms of use - on a cooperative basis/to be agreed
    b) location of the equipment and contact person (contact details) - Department of Cell Biology and Imaging, cytology and histology laboratory, Dr. Janusz Karasiński, Prof. UJ, janusz.karasinski@uj.edu.pl.

Standard equipment

• Zeiss Axiovert 40 CFL (Carl Zeiss, Germany) - bright field, phase contrast and fluorescence images of cell cultures in the excitation/emission interval for DAPI, FITC and rhodamine; AxioCam 208 color digital camera (Zeiss, Germany) supported by ZEN software 
  • a) general conditions of use - on a cooperative basis after agreement on the mode and duration of use 
    b) location of equipment and contact person (contact details): Department of Cell Biology and Imaging, cell culture laboratory, Dr. Grzegorz Tylko, Prof. UJ, grzegorz.tylko@uj.edu.pl
• Zeiss Axiovert 5 TL FL SCB (Carl Zeiss, Germany) - bright-field image registration of cell cultures, phase contrast, interference contrast and fluorescence in the excitation/emission interval for DAPI, FITC, CY3 and CY5; AxioCam 208 colour digital camera (Zeiss, Germany) operated by ZEN software; the microscope's asset is the PlasDIC interference contrast for contrast of cells from in vitro cultures grown on plastic substrates; see also
  • a) general conditions of use - on a cooperative basis after agreement on the mode and duration of use 
    b) location of equipment and contact person (contact details): Department of Cell Biology and Imaging, teaching room 0.68, Dr Grzegorz Tylko, Prof. UJ, grzegorz.tylko@uj.edu.pl
• Zeiss SteREO Discovery.V8 (Carl Zeiss, Germany) - stereomicroscope for preparation of animal and plant tissues and recording images of spatial objects in a wide field of view; working with material in transmitted light, reflected light, dark field and fluorescence for FITC and CY3; AxioCam 208 color digital camera (Zeiss, Germany) supported by ZEN software; more information
  • a) general conditions of use - on a cooperative basis after agreement on the mode and duration of use 
    b) location of equipment and contact person (contact details): Department of Cell Biology and Imaging, teaching room 0.68, Dr Grzegorz Tylko, Prof. UJ, grzegorz.tylko@uj.edu.pl.
• SpectraMax iD3 Multi-Mode Microplate Reader - Reads over a wide range of visible light, luminescence and fluorescence, i.e. Abs: 230 - 1000 nm; FL Ex: 250 - 830 nm; FL Em: 270 - 850 nm; Lumi: 300 - 850 nm. Additional functions: blending, scanning, kinetic readout (measurements over time); more details
  • a) general conditions of use - On a cooperative basis by agreement on the mode and duration of use 
    b) Location of equipment and contact person (contact details): Department of Cell Biology and Imaging, Biochemistry Laboratory, Prof. Dr. Anna M. Osyczka, anna.osyczka@uj.edu.pl
• Azure 600 gel and membrane visualisation system - visualisation of electrophoresis results on gels and membranes - chemiluminescence, fluorescence, UV, infrared 
  • a) general terms of use - on a cooperative basis/to be agreed 
    b) Location of equipment and contact person (contact details) - Department of Cell Biology and Imaging, Biochemistry Laboratory, Dr Milena Damulewicz, milena.damulewicz@uj.edu.pl.
• StepOne Real-Time PCR System (Applied Biosystems)
  • a) general conditions of use - On a cooperative basis by agreement on the mode and duration of use  
    b) Location of equipment and contact person (contact details): Department of Cell Biology and Imaging, Molecular Laboratory, Prof. Dr. Anna M. Osyczka, anna.osyczka@uj.edu.pl
• Sensoquest temperature gradient thermocycler - an instrument for standard PCR with or without temperature gradient.
  • a) general terms of use - on a collaborative/agreed basis 
    b) Location of equipment and contact person (contact details) - Department of Cell Biology and Imaging, Molecular Laboratory, Dr. Milena Damulewicz, milena.damulewicz@uj.edu.pl
• Microtome for paraffin sections (Jung Multicut 2045 Leica, Austria) - for cutting histological sections of animal and plant tissues embedded in paraffin; the microtome is accompanied by a water bath (Water Bath 1002, Histolab Products, Goteborg, Sweden).
  • a) general conditions of use - on a cooperative basis after agreement on the mode and time of use,
    b) location of equipment and contact person (contact details): Department of Cell Biology and Imaging, histology laboratory, Dr Jakub Dymek, kuba.dymek@uj.edu.pl.