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Grzegorz Tylko - Zakład Biologii i Obrazowania Komórki

Research and teaching staff

Dr hab. Grzegorz Tylko

Associate Professor of the Jagiellonian University

room 2.100 (IInd floor, C sector)

telephone: +48 12 664 53 36

e-mail: grzegorz.tylko@uj.edu.pl

ORCID: 000-0002-6669-0142

The Head of the PhD education programme “Biology” in Doctoral School of Exact and Natural Sciences

Secretary of the Faculty Team for Ensuring and Improving the Quality of Education

Member of the Program Council of the "Neurobiology" education programme

Member of the Polish Microscopy Society

Member of the Polish Cell Biology Society

Courses for PhD students

Methodology Ist year (WB.SDSP.B-1.1) (in Polish) (course coordinator)

Methodology IInd year (WB.SDSP.B-1.2) (in Polish) (course coordinator)

Seminars Ist year (WB.SDSP.B-2.1) (in Polish) (course coordinator and lecturer)

Seminars IInd year (WB.SDSP.B-2.2) (in Polish) (course coordinator)

Advances in Cell Biology (WB.SDSP.B-3) (in Polish) (course coordinator and lecturer)

Teaching activities – as part of professional practice (WB.SDSP.B-8) (in Polish) (course coordinator)

Biomedical Research Methods: From Antigen to Antibody and Beyond (WB.SDSP.PhD-3) (lecturer)

Developmental Biology (WB.SDSP.B-4) (in Polish) (course coordinator and lecturer)

Courses for students

Computer Analysis of Microscopic Images (WBL-IZ-NE/047) (lecturer)

Electron Microscopy – Applications (WBNZ-629) (course coordinator and lecturer)

Principles of Microscopy (WBNZ-214) (in Polish) (lecturer)

Proseminar (WBNZ-895) (in Polish) (lecturer)

Microscopy Techniques in Neurobiology I (WBL-IZ-NE/039) (in Polish) (course coordinator and lecturer)

Cell Biology (WBNZ-941) (in Polish) (lecturer)

Cell Biology (WBNZ-941-IK) (in Polish) (course coordinator and lecturer)

Stem Cells in In Vitro Culture with Analytical Elements (WBNZ-015) (in Polish) (lecturer)

The role of insulin and reactive oxygen species in the growth and differentiation processes of human mesenchymal stem cells. 0030/DIA/2013/42. Ministry of Science and Higher Education “Diamond Grant” for the master student Marta Dubiel

Engineering and functionalization of delivery system with Pelargonium sidoides biologically active substances in inflamed periodontal surface area. 2016/22/Z/ST5/00692. National Science Centre. UNISONO. Centre of Polymer and Carbon Materials PAN and Jagiellonian University

Long-term in vitro cultures of differentiated pre-adipocytes – the role of substrate, oxygen and crowding substances. 446.31150.4.2022. ID.UJ POB qLife MiniGrant 2022

Vacuolization of cancer cells as a stimulator of programmed cell death – introduction to radiotherapy. U1U/W18/NO/03.07. ID.UJ POB qLife MiniGrant 2024

Purchase of infrastructure for the teaching laboratory of the Faculty of Biology, Jagiellonian University. 2491. Ministry of Science and Higher Education.

Professor Grażyna Adamus, Centre for Polymer Materials of the Polish Academy of Sciences in Zabrze
Biomaterials in the form of three-dimensional scaffolds (3D) intended for regenerative medicine of soft and hard tissues. They are prepared from biodegradable organic polymers using different techniques. Their composition is variable in order to obtain optimal mechanical properties for stem cell culture followed by tissue formation. Biomaterials are also a source of active substances incorporated and released in a controlled manner. Before the scaffolds are used for tissue regeneration, they undergo biological verification in terms of their toxicity, both adhesion and migration properties for stem cells, and finally promoting or inhibiting their differentiation into the target tissue.

 

dr Andrzej Woźnica, Silesian Water Centre, University of Silesia in Katowice
For years, some countries have been struggling with the problem of mass fish kills (MFK) in inland saltwater and brackish waters. MFK is associated with the presence of the so-called golden alga, Prymnesium parvum. P. parvum is alien to inland waters, however, due to the constant discharge of salty mine waters into freshwaters, the golden alga thrives in water bodies, lakes, canals, etc. We are trying to understand the biological causes of increased mortality of organisms caused by the P. parvum bloom in order to control the course of this phenomenon. Thus, it might limit the environmental and economic effects of huge losses in the population of fish and other gill breathing organisms.

 

Professor Jacek Tomczyk, Center for Ecology and Ecophilosophy, Cardinal Stefan Wyszyński University in Warsaw
Denticles are called pulp stones, calcified forms of dental pulp. When formed in the tooth chamber, they cause pressure on the nerve and blood vessels. In the case of large denticles, they are origin of toothache. The ground of their formation has not been fully determined but it is believed that they are the effect of long-term inflammatory processes or mechanical injuries. Denticles are also found in historical teeth, derived from people living many years ago. X-ray microanalysis is the analytical method that helps to determine the elemental composition of properly developed enamel, dentine and compare it to the elemental composition of denticles. This can provide an answer to the question of the degree of mineralization of denticles formed in the teeth of contemporary and historical people. The presence of trace elements, on the other hand, may refer to the diet of ancient human populations.

Paulina Piątkowska, BSc – a first-year student of Biology. She provides a project on the optimization of the growth and differentiation of stem cells derived from adipose tissue in 3D culture conditions and in the presence of molecular crowders.
 
Lena Fus – third-year Biology student. She investigates the causes of cell vacuolization in the presence of low-molecular-weight compounds and corelates the process with endoplasmic reticulum stress.
 
Evgeniia Liutvinskaia – a second-year student of Biology works on a cellular model of osteoblastoma and the relationship between vacuolation of cell cytoplasm and methuosis in the presence of O-acylisourea

  1. Tylko G., Roomans G.M., Kilarski W. (1999) X-ray microanalysis of myotomal muscle in the sunbleak, Leucaspius delineatus (Heckel) and goldfish, Carassius auratus gibelio. Folia Biologica (Kraków) 47(3-4): 77-83. PMID: 10754791
  2. Tylko G., Kilarski W. (1999) Different methods of tissue preparation for X-ray microanalysis combined with scanning electron microscopy based on zebra fish, Brachydanio rerio muscle fibres. Folia Histochemica et Cytobiologica 37(4): 255-259. PMID: 10598327
  3. Tylko G., Karasiński J., Wróblewski R., Roomans G.M., Kilarski W. (2000) Electron probe X-ray microanalysis of cultured myogenic C2C12 cells with scanning and scanning transmission electron microscopy. Folia Histochemica et Cytobiologica 38(2): 79-84. PMID: 10833672
  4. Tylko G., Kilarski W., Mikrut M. (2002) Indirect measurement of biologically important compounds by means of X-ray microanalysis. Microchimica Acta 139(1-4): 189-193. https://doi.org/10.1007/s006040200060
  5. Tylko G., Kilarski W. (2003) Effects of Cu2+, CrO42-, Co2+ and Pb2+ on the monovalent ion content of goldfish (Carassius auratus gibelio) tissues studied by X-ray microanalysis. Folia Biologica 51(1): 125-128. PMID: 14686657
  6. Tylko G., Banach Z., Kilarski W. (2004) PROZA and calibration curves for quantitative X-ray microanalysis of biological samples. Microchimica Acta 144(4): 271-276. https://doi.org/10.1007/s00604-003-0108-z
  7. Banach Z., Borowska J., Pyza E., Tylko G. (2004) X-ray microanalysis method of elemental content evaluation in the brain of Musca domestica exposed to heavy metals. Radiation Physics and Chemistry 71(3-4): 1001-1002. https://doi.org/10.1016/j.radphyschem.2004.05.019
  8. Budka D., Mesjasz-Przybyłowicz, J., Tylko G., Przybyłowicz W. (2005) Freeze-substitution methods for Ni localization and quantitative analysis in Berkheya coddii leaves by means of PIXE. Nuclear Instruments and Methods in Physics Research B 231(1-4): 338-344. https://doi.org/10.1016/j.nimb.2005.01.080
  9. Tylko G., Banach Z., Borowska J., Niklińska M., Pyza E. (2005) Elemental changes in the brain, muscle, and gut cells of the housefly, Musca domestica, exposed to heavy metals. Microscopy Research and Technique 66(5): 239-247. https://doi.org/10.1002/jemt.20164
  10. Tylko G., Borowska J., Banach Z., Pyza E., Przybyłowicz, W.J., Mesjasz-Przybyłowicz J. (2006) Co-localization of copper, zinc and lead with calcium in their accumulation sites in the housefly's abdomen by micro-PIXE. Microchimica Acta 155(1-2): 301-304. https://doi/org/10.1007/s00604-006-0560-7
  11. Tylko G., Mesjasz-Przybyłowicz J., Przybyłowicz W.J. (2007) X-ray microanalysis of biological material in the frozen-hydrated state by PIXE. Microscopy Research and Technique 70(1): 55-68. https://doi.org/10.1002/jemt.20387
  12. Tylko G., Mesjasz-Przybyłowicz, J., Przybyłowicz W. (2007) In-vacuum micro-PIXE analysis of biological specimens in frozen-hydrated state. Nuclear Instruments and Methods in Physics Research B 260(1): 141-148. https://doi.org/10.1016/j.nimb.2007.02.017
  13. Zapotoczny S., Jurkiewicz A., Tylko G., Anielska T., Turnau K. (2007) Accumulation of copper by Acremonium pinkertoniae, a fungus isolated from industrial wastes. Microbiological Research 162(3): 219-228. https://doi.org/10.1016/j.micres.2006.03.008
  14. Płytycz B., Klimek M., Homa J., Tylko G., Kołaczkowska E. (2007) Flow cytometric measurement of neutral red accumulation in earthworm coelomocytes: novel assay for studies on heavy metal exposure. European Journal of Soil Biology 43(Supplement 1): S116-S120. https://doi.org/10.1016/j.ejsobi.2007.08.050
  15. Szczerbina T., Banach Z., Tylko G., Pyza E. (2008) Toxic effects of acrylamide on survival, development and haemocytes of Musca domestica. Food and Chemical Toxicology 46(7): 2316-2319. https://doi.org/10.1016/j.fct.2008.02.019
  16. Filipiak M., Bilska E., Tylko G., Pyza E. (2010) Effects of zinc on programmed cell death of Musca domestica and Drosophila melanogaster blood cells. Journal of Insect Physiology 56(4): 383-390. https://doi.org/10.1016/j.jinsphys.2009.11.010
  17. Janik P., Tylko G., Ostachowicz B., Turnau K. (2010) Elemental composition of Physarum compressum Alb. et Schw. sporocarps and their structures cultivated on rabbit dung and agar substrates. Microscopy Research and Technique 73(12): 1134-1142. https://doi.org/10.1002/jemt.20860
  18. Lenartowicz M., Windak R., Tylko G., Kowal M., Styrna J. (2010) Effects of copper supplementation on the structure and content of elements in kidneys of Mosaic mutant mice. Biological Trace Element Research 136(2): 204-220. https://doi.org/10.1007/s12011-009-8533-4
  19. Szostek K., Stepańczak B., Szczepanek A., Kępa M., Głąb H., Jarosz P., Włodarczyk P., Tunia K., Pawlyta J., Paluszkiewicz C., Tylko G. (2011) Diagenetic signals from ancient human remains – bioarcheological applications. Mineralogia 42(2-3): 93-112. https://doi.org/10.2478/v10002-011-0009-4
  20. Stępień E., Miszalski-Jamka T., Kapusta P., Tylko G., Pasowicz M. (2011) Beneficial effect of cigarette smoking cessation on fibrin clot properties. Journal of Thrombosis and Thrombolysis 32(2): 177-182. https://doi.org/10.1007/s11239-011-0593-6
  21. Słomka A., Kuta E., Szarek-Łukaszewska G., Godzik B., Kapusta P., Tylko G., Bothe H. (2011) Violets of the section Melanium, their colonization by arbuscular mycorrhizal fungi and their occurrence on heavy metal heaps. Journal of Plant Physiology 168(11): 1191-1199. https://doi.org/10.1016/j.jplph.2011.01.033
  22. Pamuła E., Kokoszka J., Cholewa-Kowalska K., Łączka M., Kantor Ł., Niedźwiedzki Ł., Reilly G.C., Filipowska J., Madej W., Kołodziejczyk M., Tylko G., Osyczka A.M. (2011) Degradation, bioactivity, and osteogenic potential of composites made of PLGA and two different sol-gel bioactive glasses. Annals of Biomedical Engineering 39(8): 2114-2129. https://doi.org/10.1007/s10439-011-0307-4
  23. Filipiak M., Tylko G., Kilarski W. (2012) Flow cytometric determination of genome size in European sunbleak Leucaspius delineatus (Heckel, 1843). Fish Physiology and Biochemistry 38(2): 355-362. https://doi.org/10.1007/s10695-011-9512-1
  24. Szewczyk Ł., Ulańska J., Dubiel M., Osyczka A.M., Tylko G. (2012) The effect of acrylamide and nitric oxide donors on human mesenchymal progenitor cells. Toxicology In Vitro 26(6): 897-906. https://doi.org/10.1016/j.tiv.2012.04.016
  25. Filipak M., Tylko G., Pyza E. (2012) Zinc induces caspase-dependent mitochondrial pathway of the programmed cell death in haemocytes of Drosophila melanogaster. Biometals 25(3): 507-516. https://doi.org/10.1007/s10534-012-9530-1
  26. Stępień E., Kabłak-Ziembicka A., Musiałek P., Tylko G., Przewłocki T. (2012) Fibrinogen and carotid intima media thickness determine fibrin density in different arteriosclerosis extents. International Journal of Cardiology 157(3): 411-413. https://doi.org/10.1016/j.ijcard.2012.03.140
  27. Krząkała A., Służalska K., Dercz G., Maciej A., Kazek A., Szade J., Winiarski, A., Dudek M., Michalska J., Tylko G., Osyczka A.M., Simka W. (2012) Characterization of bioactive films on Ti-6Al-4V alloy. Electrochimica Acta 104: 425-438. https://doi.org/10.1016/j.electacta.2012.12.081
  28. Krząkała A., Służalska K., Widziołek M., Szade J., Winiarski, A., Dercz G., Kazek A., Tylko G., Michalska J., Iwaniak A., Osyczka A.M., Simka W. (2012) Formation of bioactive coatings on a Ti-6Al-7Nb alloy by plasma electrolytic oxidation. Electrochimica Acta 104: 407-424. https://doi.org/10.1016/j.electacta.2012.07.075
  29. Wang Y.D., Mesjasz-Przybyłowicz J, Tylko G, Barnabas A.D., Przybyłowicz W.J. (2013) Micro-PIXE analyses of frozen-hydrated semi-thick biological sections. Nuclear Instruments and Methods in Physics Research Section B 306: 134-139. https://doi.org/10.1016/j.nimb.2012.12.051
  30. Tylko G. (2013) Analysis of biologically-derived small particles. Searching for geometry correction factors using Monte Carlo simulation. Microscopy and Microanalysis 19(1): 56-65. https://doi.org/10.1017/S1431927612013803
  31. Babilas D., Służalska K., Krząkała A., Maciej A., Socha R.P., Dercz G., Tylko G., Michalska J., Osyczka A.M., Simka W. (2013) Plasma electrolytic oxidation of a Ti-15Mo alloy in silicate solutions. Materials Letters 100: 252-256. https://doi.org/10.1016/j.matlet.2013.03.047
  32. Augustynowicz J., Wróbel P., Płachno B.J., Tylko G., Gajewski Z., Węgrzynek D. (2014) Chromium distribution in shoots of macrophyte Callitriche cophocarpa Sendtn. Planta 239(6): 1233-1242. https://doi.org/10.1007/s00425-014-2047-9
  33. Filipowska J., Pawlik J., Cholewa-Kowalska K., Tylko G., Pamuła E., Niedźwiedzki Ł., Szuta M., Łączka M., Osyczka A.M. (2014) Incorporation of sol-gel bioactive glass into PLGA improves mechanical properties and bioactivity of composite scaffolds and results in their osteoinductive properties. Biomedical Materials 9 (6): 065001. https://doi.org/10.1088/1748-6041/9/6/065001
  34. Dubiel M., Lasota J., Tylko G., Osyczka A.M. (2015) Role of BMP signaling pathways in osteogenesis. Postępy Biologii Komórki 42(4): 645-665. https://www.pbkom.eu/pl/content/rola-%C5%9Bcie%C5%BCek-sygnalizacji-bmp-w-procesach-osteogenezy
  35. Sowa M., Piotrowska M., Widziołek M., Dercz G., Tylko G., Gorewoda T., Osyczka A.M. Simka W. (2015) Bioactivity of coatings formed on Ti-13Nb-13Zr alloy using plasma electrolytic oxidation. Materials Science & Engineering C – Materials for Biological Applications 49: 159-173. https://doi.org/10.1016/j.msec.2014.12.073
  36. Ogar A., Tylko G., Turnau K. (2015) Antifungal properties of silver nanoparticles against indoor mould growth. Science of the Total Environment 521-522: 305-314. https://doi.org/10.1016/j.scitotenv.2015.03.101
  37. Tylko G. (2016) Cell on biomaterials – some aspects of elemental analysis by means of electron probes. Journal of Microscopy 261(2): 185-195. https://doi.org/10.1111/jmi.12318
  38. Kula J., Błasiak A., Czerw A., Tylko G., Sowa J., Hess G. (2016) Short-term repeated corticosterone administration enhances glutamatergic but not GABAergic transmission in the rat motor cortex. Pflugers Archiv European Journal of Physiology 468(4): 679-691. https://doi.org/10.1007/s00424-015-1773-6
  39. Kazek-Kęsik A., Kuna K., Dec W., Widziołek M., Tylko G., Osyczka A.M., Simka W. (2016) In vitro bioactivity investigations of Ti-15Mo alloy after electrochemical surface modification. Journal of Biomedical Materials Research Part B – Applied Biomaterials 104 (5): 903-913. https://doi.org/10.1002/jbm.b.33442
  40. Kula J., Guguła A., Błasiak A., Bobula B., Danielewicz J., Kania A., Tylko G., Hess G. (2017) Diverse action of repeated corticosterone treatment on synaptic transmission, neuronal plasticity, and morphology in superficial and deep layers of the rat motor cortex. Pflugers Archiv European Journal of Physiology 469(11): 1519-1532. https://doi.org/10.1007/s00424-017-2036-5
  41. Stępień E.Ł., Durak-Kozica M., Kamińska A., Targosz-Korecka M., Libera M., Tylko G., Opalińska A., Kapusta M., Solnica B., Georgescu A., Costa M.C., Czyżewska-Buczyńska A., Witkiewicz W., Malecki M.T., Enguita F.J. (2018) Circulating ectosomes: determination of angiogenic microRNAs in type 2 diabetes. Theranostics 8(14): 3874-3890. https://doi.org/10.7150/thno.23334
  42. van der Ent A., Przybyłowicz W.J., de Jonge M.D., Harris H.H., Ryan C.G., Tylko G., Paterson D.J., Barnabas A.D., Kopittke P.M., Mesjasz-Przybyłowicz J. (2018) X-ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants. New Phytologist 218(2): 432-452. https://doi.org/10.1111/nph.14810
  43. Ryszka P., Lichtscheidl I., Tylko G., Turnau K. (2019) Symbiotic microbes of Saxifraga stellaris ssp. alpigena from the copper creek of Schwarzwand (Austrian Alps) enhance plant tolerance to copper. Chemosphere 228: 183-194. https://doi.org/10.1016/j.chemosphere.2019.04.084
  44. Michalska J., Sowa M., Piotrowska M., Widziołek M., Tylko G., Dercz G., Socha R.P., Osyczka A.M., Simka W. (2019) Incorporation of Ca ions into anodic oxide coatings on the Ti-13Nb-13Zr alloy by plasma electrolytic oxidation. Materials Science & Engineering C – Materials for Biological Applications 104: 109957. https://doi.org/10.1016/j.msec.2019.109957
  45. Majerczak J., Filipowska J., Tylko G., Guzik M., Karasiński J., Piechowicz E., Pyza E., Chłopicki S., Żołądź J.A. (2020) Impact of long-lasting spontaneous physical activity on bone morphogenetic protein 4 in the heart and tibia in murine model of heart failure. Physiological Reports 8(8): e14412. https://doi.org/10.14814/phy2.14412
  46. Spyrka J., Gugula A., Rak A., Tylko G., Hess G., Blasiak A. (2020) Early life stress-induced alterations in the activity and morphology of ventral tegmental area neurons in female rats. Neurobiology of Stress 13: 100250. https://doi.org/10.1016/j.ynstr.2020.100250
  47. Turnau K., Fiałkowska E., Ważny R., Rozpądek P., Tylko G., Bloch S., Nejman-Faleńczyk B., Grabski M., Węgrzyn A., Węgrzyn G. (2021) Extraordinary multi-organismal interactions involving bacteriophages, bacteria, fungi, and rotifers: quadruple microbial trophic network in water droplets. International Journal of Molecular Sciences 22: 2178. https://doi.org/10.3390/ijms22042178
  48. Pańczyszyn E., Jaśko M., Miłek O., Niedziela M., Męcik-Kronenberg T., Hoang-Bujnowicz A., Zięba M., Adamus G., Kowalczuk M., Osyczka A.M., Tylko G. (2021) Gellan gum hydrogels cross-linked with carbodiimide stimulates vacuolation of human tooth-derived stem cells in vitro. Toxicology In Vitro 73: 105111. https://doi.org/10.1016/j.tiv.2021.105111
  49. Tuwalska A., Sionkowska A., Bryła A., Tylko G., Osyczka A.M., Laus M., Vojtova L. (2022) A biological study of composites based on the blends of nanohydroxyapatite, silk fibroin and chitosan. Materials 15(15): 5444. https://doi.org/10.3390/ma15155444
  50. Gugula A., Trenk A., Celary A., Cizio K., Tylko G., Błasiak A., Hess G. (2022) Early-life stress modifies the reactivity of neurons in the ventral tegmental area and lateral hypothalamus to acute stress in female rats. Neuroscience 490: 49-65. https://doi.org/10.1016/j.neuroscience.2022.02.017
  51. Chaber P., Tylko G., Włodarczyk J., Nitschke P., Hercog A., Jurczyk S., Rech J., Kubacki J., Adamus G. (2022) Surface modification of PHBV fibrous scaffold via lithium borohydride reduction. Materials 15: 7494. https://doi.org/10.3390/ma15217494
  52. Al Abaquita T.L., Damulewicz M., Tylko G., Pyza E. (2023) The dual role of heme oxygenase in regulating apoptosis in the nervous system of Drosophila melanogaster. Frontiers in Physiology 14. https://doi.org/10.3389/fphys.2023.1060175
  53. Zięba M., Sikorska W., Musioł M., Janeczek H., Włodarczyk J., Pastusiak M., Gupta A., Radecka I., Parati M., Tylko G., Kowalczuk M., Adamus, G. (2024) Designing of drug delivery systems to improve the antimicrobial efficacy in the periodontal pocket based on biodegradable polyesters. International Journal of Molecular Sciences, 25(1): 503. https://doi.org/10.3390/ijms25010503
  54. Woźnica, A., Karczewski, J., Lipowczan, M., Tylko, G., Jarosz, W., Matysik, M., Sierka, M., Janczewska, N., Bąk, M., Prokopowicz, A., Robaszkiewicz, E., Libera, M., Pasztaleniec, A., Kolada, A., Mazur-Marzec, H., Absalon, D. (2024) The reaction of Prymnesium parvum to a sudden salinity decrease. Ecohydrology & Hydrobiology. https://doi.org/10.1016/j.ecohyd.2024.10.004
  55. Milenin, A., Niedźwiedzki, Ł., Truchan, K., Guzik, G., Kąc, S., Tylko, G., Osyczka, A. M. (2024) Investigating the anticancer potential of zinc and magnesium alloys: From base materials to nanocoated titanium implants. Materials 17(13): 3365. https://doi.org/10.3390/ma17133365
  56. Surman, M., Wilczak, M., Bzowska, M., Tylko, G., Przybyło, M. (2024) The proangiogenic effects of melanoma-derived ectosomes are mediated by αvβ5 integrin rather than αvβ3 integrin. Cells 13(16): 1336. https://doi.org/10.3390/cells13161336
  57. Górska-Andrzejak, J., Widacha, L., Wadowski, R., Mitka, M., Tylko, G. (2024) Dietary acrylamide disrupts the functioning of the biological clock. Journal of Hazardous Materials 476: 134912. https://doi.org/10.1016/j.jhazmat.2024.134912
  58. Duda, M., Maciak, P., Tylko, G., Wartalski, K., Trzcińska, M., Romek, M., Samiec, M. (2024) Research note: Harnessing the potential of chicken blastodermal cells as a new frontier in poultry science and biotechnology – Establishment of embryonic stem cells and their differentiability towards Schwann cell-like cell lineages. Poultry Science 104(2): 104701. https://doi.org/10.1016/j.psj.2024.104701

  1. Tylko G., Roomans G.M., Kilarski W. (1999) X-ray microanalysis of myotomal muscle in the sunbleak, Leucaspius delineatus (Heckel) and goldfish, Carassius auratus gibelio. Folia Biologica (Kraków) 47(3-4): 77-83. PMID: 10754791
  2. Tylko G., Kilarski W. (1999) Different methods of tissue preparation for X-ray microanalysis combined with scanning electron microscopy based on zebra fish, Brachydanio rerio muscle fibres. Folia Histochemica et Cytobiologica 37(4): 255-259. PMID: 10598327
  3. Tylko G., Karasiński J., Wróblewski R., Roomans G.M., Kilarski W. (2000) Electron probe X-ray microanalysis of cultured myogenic C2C12 cells with scanning and scanning transmission electron microscopy. Folia Histochemica et Cytobiologica 38(2): 79-84. PMID: 10833672
  4. Tylko G., Kilarski W., Mikrut M. (2002) Indirect measurement of biologically important compounds by means of X-ray microanalysis. Microchimica Acta 139(1-4): 189-193. https://doi.org/10.1007/s006040200060
  5. Tylko G., Kilarski W. (2003) Effects of Cu2+, CrO42-, Co2+ and Pb2+ on the monovalent ion content of goldfish (Carassius auratus gibelio) tissues studied by X-ray microanalysis. Folia Biologica 51(1): 125-128. PMID: 14686657
  6. Tylko G., Banach Z., Kilarski W. (2004) PROZA and calibration curves for quantitative X-ray microanalysis of biological samples. Microchimica Acta 144(4): 271-276. https://doi.org/10.1007/s00604-003-0108-z
  7. Banach Z., Borowska J., Pyza E., Tylko G. (2004) X-ray microanalysis method of elemental content evaluation in the brain of Musca domestica exposed to heavy metals. Radiation Physics and Chemistry 71(3-4): 1001-1002. https://doi.org/10.1016/j.radphyschem.2004.05.019
  8. Budka D., Mesjasz-Przybyłowicz, J., Tylko G., Przybyłowicz W. (2005) Freeze-substitution methods for Ni localization and quantitative analysis in Berkheya coddii leaves by means of PIXE. Nuclear Instruments and Methods in Physics Research B 231(1-4): 338-344. https://doi.org/10.1016/j.nimb.2005.01.080
  9. Tylko G., Banach Z., Borowska J., Niklińska M., Pyza E. (2005) Elemental changes in the brain, muscle, and gut cells of the housefly, Musca domestica, exposed to heavy metals. Microscopy Research and Technique 66(5): 239-247. https://doi.org/10.1002/jemt.20164
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